Abstract
The work presents a comprehensive selection of conditions for the synthesis of nanosized Copper particles in an aqueous oxidizing environment using a biocompatible amino acid – L-Сysteine as a stabilizer, a reducing agent – sodium tetraborate, and the application of the method of mathematical experimental planning the Scheffe method.
The use of the mathematical planning method made it possible to predict the additive effect of the ratio between precursors in the studied medium on the value of the optical
absorption edge of the obtained colloidal solutions of copper nanoparticles, their stability over time and the effect on test cultures of microorganisms P.aeruginosa, C.albicans, B.subtilitis.
The ratio between the starting reagents that lead to the formation of stable colloidal solutions of copper nanoparticles at pH=6 and temperature of 20°C in an oxidizing reaction medium has been established.
A mathematical model was constructed in the form of a projection onto the plane of an equilateral triangle of the dependence of the value of the optical absorption edge of colloidal solutions of metallic copper nanoparticles on the ratio between the precursors. A mathematical equation was obtained – a fourth-degree polynomial that describes the dependence of the value of the optical absorption edge of colloidal solutions of copper nanoparticles on the ratio between three independent variables – crystal-forming components of time-stable particles in the reaction medium.
The antibacterial activities of a series of test solutions were investigated by the micromethod of serial dilutions in accordance with the procedures of the European Committee for Susceptibility Testing against reference strains of bacteria (P.aeruginosa, C.albicans, B.subtilitis.). Using the Scheffe mathematical model, the concentration regions and ratios between the components of the studied system were determined, which had the highest impact on the action of test cultures of microorganisms.
References
Telegeeva P.G., Efremenko D.S., Telegeev G.D., Malyuta S.S. Application of nanoparticles in biomedicine. Biotechnologia acta. 2013. 6(2): 21–32. (in Ukrainian)
https://doi.org/10.15407/biotech6.02.021
Andrusyshina I.N. Nanoparticles of metals, production methods, physical and chemical properties, research methods and toxicity assessment. Modern problems of toxicology. 2011. 3: 5–14. (in Ukrainian)
http://protox.medved.kiev.ua/index.php/ua/categories/problems-articles/item/93-metal- nanoparticles-types-of-production-physicochemical-properties-methods-of-investigation-and-toxicity-assessment
Irwin P., Martin J., Nguyen LH. He Y., Geh¬ring А., Chen C.Y. Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase. J Nanobiotechnol. 2010. 8(34): 1–12.
https://doi.org/10.1186/1477-3155-8-34
Trakhtenberg I.M., Dmytrukha N.M. Principles. Methods and indicators of experimental assessment of the safety of metal nanopar¬ticles. Ukrainian Journal of Modern Problems of Toxicology. 2016. 4: 5–17. (in Ukrainian).
Pal S., Tak Y., Song J.M. Antimicrobial activity of spherical silver nanoparticles prepared using a biocompatible macromolecular capping agent: evidence for induction of a greatly prolonged bacterial lag phase. Journal of Nanobiotechnology. 2010. 8(34): 1–12.
https://link.springer.com/article/10.1186/1477- 3155-8-34
Brigger I., Dubernet C., Couvreur P. Nanoparticles in cancer therapy and diagnosis. Adv. Drug Deliv. Rev. 2002. 54: 631–65.
https://doi.org/10.1016/S0169-409X(02)000 44-3
Trakhtenberg I.M., Dmytrukha N.M. Nanoscale metals, methods of production, areas of application, physicochemical and toxic properties. Ukrainian Journal of Occupational Medicine. 2013. 4(37): 62–74. (in Ukrainian).
http://nbuv.gov.ua/UJRN/Ujpmp_2013_4_8
Mykytyuk M.V. Nanoparticles and prospects for their application in biology and medicine. Problems of ecology and medicine. 2011. 15(5–6): 41–49. (in Ukrainian).
http://nbuv.gov.ua/UJRN/petm_2011_15_5-6_10
Petrik I. S., Eremenko A. M., Smirnova N. P., Korchak G. I., Mikhiyenkova A. I. Synthesis and Stabilization of Cu Nanoparticles in Aqueous Solutions and Their Bactericidal Activity. Him. Fiz. Tehnol. Poverhni. 2014. 5: 74–81.
https://www.cpts.com.ua/index.php/cpts/article/view/264
Zhang Q. Preparation of copper nanoparticles by chemical reduction method using potassium dorohydride. Trans. Nonferrous Met. Soc. China 2010. 20: 240–244.
https://doi.org/10.1016/S1003-6326(10)6004 7-7
Aguilar M. S., Esparza R., Rosas G. Synthesis of Cu nanoparticles by chemical reduction method. Transactions of Nonferrous Metals Society of China. 2019. 29(7): 1510–1515.
https://doi.org/10.1016/S1003-6326(19)6505 8-2
Shiv Shankar, Jong-Whan Rhim. Effect of copper salts and reducing agents on characteristics and antimicrobial activity of copper nanoparticles. Materials Letters. 2014. 132: 307–311.
https://doi.org/10.1016/J.MATLET.2014.06. 014
Gruzina T. G., Rieznichenko L. S., Yakubenko L. M., Podolska V. I., Grishchenko N. I., Ulberg Z. R., Dybkova S. M. Study of the toxic effect of copper nanoparticles: effect on electrosurface and biochemical parameters of bacterial cells. Chemistry, Physics and Technology of Surface. 2023. 14(3): 372–382. (in Ukrainian).
https://doi.org/10.15407/hftp14.03.372
Palaniselvam Kuppusamy, Mashitah M. Yusoff, Gaanty Pragas Maniam, Natanamurugaraj Govindan. Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications – An updated report. Saudi Pharmaceutical Journal. 2016. 24(4): 473–484.
https://doi.org/10.1016/j.jsps.2014.11.013
Swathi Pavithran, Manikantan Pappuswamy, Yamuna Annadurai, Vijaya Anand Armugam & Thirunavukkarasu Periyaswamy. Green Synthesis of Copper Nanoparticles, Characterization and Their Applications. J. Appl. Life Sci. Int. 2020. 23: 10–24.
https://doi.org/10.9734/jalsi/2020/v23i730172
Michaela Corina Crisan, Mocan Teodora, Mocan Lucian. Copper Nanoparticles: Synthesis and Characterization, Physiology, Toxicity and Antimicrobial Applications. Appl. Sci. 2022. 12(1): 141.
https://doi.org/10.3390/app12010141
Stravskyy Y. S., Fedoniuk Ya., Yarema O. M., Reznichenko S. Preclinical Studies of Copper Nanoparticles. Medical and Clinical Chemistry. 2020. 22(3): 99–106. (in Ukrainian).
https://doi.org/10.11603/mcch.2410-681X. 2020.v.i3.11546
Petrik I. S., Eremenko A. M., Smirnova N. P., Marinin A. I., Olishevsky V. V. Pecularities of Copper and Silver Nanoparticles Stabilization by Cysteine in Aqueous Colloidal Systems. Chemistry, Physics and Technology of Surface. 2015. 6(3): 364–371. (in Ukrainian).
https://doi.org/10.15407/hftp06.03.364
Krupko O. V., Khalavka Yu. B., Shcherbak L. P. Synthesisof CdS/L-Сys nanoparticles colloid solutions with predetermined optical pro¬perties. Materials Research Bulletin. 2014. 60: 264–269. https://doi.org/10.1016/j.materresbull.2014.08.026